diff --git a/Lib/doctest.py b/Lib/doctest.py
index 114aac62a34e95..44068f94d9b90e 100644
--- a/Lib/doctest.py
+++ b/Lib/doctest.py
@@ -2225,13 +2225,13 @@ def __init__(self, test, optionflags=0, setUp=None, tearDown=None,
         unittest.TestCase.__init__(self)
         self._dt_optionflags = optionflags
         self._dt_checker = checker
-        self._dt_globs = test.globs.copy()
         self._dt_test = test
         self._dt_setUp = setUp
         self._dt_tearDown = tearDown
 
     def setUp(self):
         test = self._dt_test
+        self._dt_globs = test.globs.copy()
 
         if self._dt_setUp is not None:
             self._dt_setUp(test)