Here is a code example that shows issue.
When removing "self.canvas1.set_window_title('Title Test')" program runs with no errors.

# -*- coding: utf-8 -*-

from PyQt5 import QtCore, QtGui, QtWidgets

import matplotlib as mpl
import matplotlib.pyplot as plt
from matplotlib.backends.backend_qt5agg import FigureCanvasQTAgg as FigureCanvas
from matplotlib.backends.backend_qt5agg import NavigationToolbar2QT as NavigationToolbar
from PyQt5 import QtCore


class Ui_Form(object):

    def setupUi(self, Form):
        Form.setObjectName("Form")
        Form.resize(745, 556)
        self.verticalLayout = QtWidgets.QVBoxLayout(Form)
        self.verticalLayout.setObjectName("verticalLayout")
        QtCore.QMetaObject.connectSlotsByName(Form)

        #
        self.setup_plot()

    def setup_plot(self):
        dpi = 75
        fig1 = plt.figure(dpi=dpi, facecolor='#617f8a', tight_layout=True)
        mpl.rc('axes', facecolor='#ffffff')
        self.plot1 = fig1.add_subplot(111)
        self.canvas1 = FigureCanvas(fig1)
        self.verticalLayout.addWidget(self.canvas1)
        toolbar = NavigationToolbar(self.canvas1, self.canvas1)
        toolbar.setMaximumSize(QtCore.QSize(16777215, 35))
        self.verticalLayout.addWidget(toolbar)
        self.canvas1.set_window_title('Title Test')


if __name__ == "__main__":
    import sys
    app = QtWidgets.QApplication(sys.argv)
    Form = QtWidgets.QWidget()
    ui = Ui_Form()
    ui.setupUi(Form)
    Form.show()
    sys.exit(app.exec_())

[@tacaswell edited to add multi-line markup]

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