Genotyping and Phylogenetic Analysis of the Major Genes in Respiratory Syncytial Virus Isolated from Infants with Bronchiolitis

Original Articles

Kazuko Goto-Sugai, Hiroyuki Tsukagoshi, Katsumi Mizuta, Shunji Matsuda, Masahiro Noda, Toshiyuki Sugai, Yoshihiro Saito, Nobuhiko Okabe, Masato Tashiro, Kunihisa Kozawa, Ryota Tanaka, Yukio Morita, Atsuyoshi Nishina, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Japan">Hirokazu Kimura

Author information

Kazuko Goto-Sugai
National Hospital Organization Yokohama Medical Center, Japan
Hiroyuki Tsukagoshi
Gunma Prefectural Institute of Public Health and Environmental Sciences, Japan
Katsumi Mizuta
Yamagata Prefectural Institute of Public Health, Japan
Shunji Matsuda
National Hospital Organization Ehime National Hospital, Japan
Masahiro Noda
Department of Virology III, National Institute of Infectious Diseases, Japan
Toshiyuki Sugai
Department of Virology III, National Institute of Infectious Diseases, Japan
Yoshihiro Saito
Department of Pediatrics, Jikei University School of Medicine, Japan
Nobuhiko Okabe
Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Japan
Masato Tashiro
Influenza Virus Research Center, National Institute of Infectious Diseases, Japan
Kunihisa Kozawa
Gunma Prefectural Institute of Public Health and Environmental Sciences, Japan
Ryota Tanaka
Department of Surgery, Institute of Medical Sciences, Kyorin University, Japan
Yukio Morita
Gunma Prefectural Institute of Public Health and Environmental Sciences, Japan
Atsuyoshi Nishina
Yamagata Prefectural Yonezawa Women's Junior College, Japan
Hirokazu Kimura
Gunma Prefectural Institute of Public Health and Environmental Sciences, Japan
Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Japan

JOURNAL FREE ACCESS

2010 Volume 63 Issue 6 Pages 393-400

DOI https://doi.org/10.7883/yoken.63.393

Details

Abstract

We performed the genotyping and phylogenetic analysis of respiratory syncytial virus (RSV) isolated from 17 infants with bronchiolitis in Kanagawa Prefecture, Japan in 2005 and 2006. The major genes in these samples (attachment [G] glycoprotein gene, fusion [F] protein gene, and nucleoprotein [N] gene) were sequenced and analyzed genetically. Phylogenetic analysis of these genes revealed that 7 and 10 strains could be classified into subgroups A and B, respectively. Phylogenetic analysis of the G gene revealed that the subgroup A and B strains were unique genotypes GA2 and BA, respectively. Moreover, the amino acid sequences for these genotypes suggested a relatively high frequency of amino acid substitutions in the G and F proteins in these strains, whereas the N protein was highly homologous. These results suggest that RSV genotypes GA2 and BA may be associated with bronchiolitis in the cases studied here.

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