Abstract
Breast cancer (BC) is the most frequent cancer and the leading cause of cancer-related mortality in women. The treatment techniques for the BC include chemotherapy (CT) and/or radiotherapy (RT) and can modify elementary the cell matrix by calcificating tissues due to biological and morphological changes. Also, treatments for BC induce cardiotoxicity and it is important to understand the mechanisms involved in order to prevent this late effect in treated breast cancer patients. The high incidence of cardiovascular mortality in breast cancer patients is partially credited to increased intimal and medial calcifications of the aorta. The aim of this work is to investigate the distibution of low atomic number elements such as Magnesium (Mg), due to its importance for the cardiac metabolism; iron (Fe), since BC treatment may be associated with oxidative stress; and Sodium (Na), that is extremely related to the damage of endothelial cells. An optimal technique to observe these changes in aorta tissue is soft X-ray FLuorescence that can provide elemental maps of these important elements. The results performed by Low Energy X-ray Fluorescence LEXRF analyses showed that when the tissue is submitted to treatments with CT and/or RT, some normal structures become disorganized, and consequently the intensity of elemental compounds can be changed. All the experiments were carried out at the TwinMic beamline at Elettra Synchrotron facility using as animal model Wistar rats in order to evaluate the distribution of Na, Mg and Fe in aorta walls of Wistar rats, after BC treatment. Simultaneous acquisition of LEXRF and attenuation coefficient maps suggest that the combined chemotherapy and radiotherapy caused more damage to the aortic tissue as compared to radiation therapy alone. These findings add an in-depth understanding of elemental lack or excess in the tissue and contribute to locate these changes.