The Karonda, (Carissa carandas), belongs to family Apocynaceae. It is a sprawling semi-vine shrub... more The Karonda, (Carissa carandas), belongs to family Apocynaceae. It is a sprawling semi-vine shrub native to India. Being thorny, it is used as live fencing around the fields besides providing tasty fruits. Karonda fruit is used in various preparations and these are increase possibilities for processing. It has remained largely unexploited, Therefore, a study was conducted at Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow. Thirty elite genotypes were identified from Mirzapur, Varansi, Faizabad and Lucknow districts of U.P Vengurla, Belgaun and Gokak area of Karnataka, Ajmer, Chittorgarh and Siroli area of Rajasthan and Neemach (M.P). Floral characterization revealed flowering period was from March to April with full bloom in April first Week under Lucknow conditions. Anthesis was recorded after 1.00 pm, followed with anther dehiscence after few hours. Poor pollen fertility and germinability suggests scope of improvement in the crop yield.The mature fruits analyzed for fruit weight, length, diameter, pulp and seed content, TSS (oBrix) acidity and ascorbic acid content revealed that there was wide variation among the accessions. The fruit colour was purple and red-white; fruit weight ranged from 1.85 to 6.0 g; fruit length from 0.90 to 2.40 cm; diameter from 0.32 to 2.15 cm; seed weight from 0.26 to 1.40 g and pulp content from 1.35 to 4.67 g; Total Soluble solids (oBrix) in different accessions varied from 5.20 to 9.50, Titrable acidity varied from 0.45 to 2.91% whereas Ascorbic acid content varied from 23.20 to 31.46 mg/100g. On the basis of physico-chemical characters of fruits, 3 accessions viz. CISH Kr-10, CISH Kr-11 and CISH Kr-12 have been identified for multiplication and further evaluation.
Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to a... more Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to id...
Proteome-based vegetative and flower bud formation characterization was utilized to identify and ... more Proteome-based vegetative and flower bud formation characterization was utilized to identify and differentiate protein species with significant variable abundance during floral transition in mango cv. Dashehari using 2DE and corroborated the identified protein spots using gene expression analysis. Total soluble proteins were phenol-extracted from mango cv's vegetative and floral flush. Dashehari and separated on 2D gels at pH 4-7. The protein spots with variable intensity were identified through SameSpots software. The protein sequences of differentially accumulated spots were identified based on PI and MW using Citrus sinensis proteome isoelectric focusing database. Furthermore, these protein sequences were used to conduct (tBLASTn) against Mangifera indica to predict the protein. Real time gene expression was done to corroborate identified proteins. Total 301 spots were detected, out of which 16 were identified as differentially expressed (P?0.05) and a 2-fold change. These 16 protein spots were identified on the basis of in silico comparative mapping protein against genome of mango and citrus, a close relative. They were classified into eight categories: transcriptional regulation, phenylpropanoid pathway and cell wall /cytoskeleton metabolism-related proteins, hormone signalling, flowering time, signal-transduction, transport and protein synthesis to flowering. Five genes coding for shortlisted proteins were used for validation of results using gene expression analysis. SAM (S adenosyl methonine synthase) was found up-regulated in floral flush, involved in the biosynthesis of polyamines has association with flowering, and stress responses. Furthermore, ARF (Auxin Response Factor), serine/threonine kinase gene members were also found to play critical role in determining floral development process in mango, consistent with results obtained through 2DE. Protein species that are putatively involved in phenylpropanoid pathway were also identified, showing the process of mango flowering from a new perspective beyond the conventional view. This flowering related proteomics study provides an overview of the biological pathways and regulatory mechanisms associated with flowering developmental physiology.
The Karonda, (Carissa carandas), belongs to family Apocynaceae. It is a sprawling semi-vine shrub... more The Karonda, (Carissa carandas), belongs to family Apocynaceae. It is a sprawling semi-vine shrub native to India. Being thorny, it is used as live fencing around the fields besides providing tasty fruits. Karonda fruit is used in various preparations and these are increase possibilities for processing. It has remained largely unexploited, Therefore, a study was conducted at Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow. Thirty elite genotypes were identified from Mirzapur, Varansi, Faizabad and Lucknow districts of U.P Vengurla, Belgaun and Gokak area of Karnataka, Ajmer, Chittorgarh and Siroli area of Rajasthan and Neemach (M.P). Floral characterization revealed flowering period was from March to April with full bloom in April first Week under Lucknow conditions. Anthesis was recorded after 1.00 pm, followed with anther dehiscence after few hours. Poor pollen fertility and germinability suggests scope of improvement in the crop yield.The mature fruits analyzed for fruit weight, length, diameter, pulp and seed content, TSS (oBrix) acidity and ascorbic acid content revealed that there was wide variation among the accessions. The fruit colour was purple and red-white; fruit weight ranged from 1.85 to 6.0 g; fruit length from 0.90 to 2.40 cm; diameter from 0.32 to 2.15 cm; seed weight from 0.26 to 1.40 g and pulp content from 1.35 to 4.67 g; Total Soluble solids (oBrix) in different accessions varied from 5.20 to 9.50, Titrable acidity varied from 0.45 to 2.91% whereas Ascorbic acid content varied from 23.20 to 31.46 mg/100g. On the basis of physico-chemical characters of fruits, 3 accessions viz. CISH Kr-10, CISH Kr-11 and CISH Kr-12 have been identified for multiplication and further evaluation.
Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to a... more Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to id...
Proteome-based vegetative and flower bud formation characterization was utilized to identify and ... more Proteome-based vegetative and flower bud formation characterization was utilized to identify and differentiate protein species with significant variable abundance during floral transition in mango cv. Dashehari using 2DE and corroborated the identified protein spots using gene expression analysis. Total soluble proteins were phenol-extracted from mango cv's vegetative and floral flush. Dashehari and separated on 2D gels at pH 4-7. The protein spots with variable intensity were identified through SameSpots software. The protein sequences of differentially accumulated spots were identified based on PI and MW using Citrus sinensis proteome isoelectric focusing database. Furthermore, these protein sequences were used to conduct (tBLASTn) against Mangifera indica to predict the protein. Real time gene expression was done to corroborate identified proteins. Total 301 spots were detected, out of which 16 were identified as differentially expressed (P?0.05) and a 2-fold change. These 16 protein spots were identified on the basis of in silico comparative mapping protein against genome of mango and citrus, a close relative. They were classified into eight categories: transcriptional regulation, phenylpropanoid pathway and cell wall /cytoskeleton metabolism-related proteins, hormone signalling, flowering time, signal-transduction, transport and protein synthesis to flowering. Five genes coding for shortlisted proteins were used for validation of results using gene expression analysis. SAM (S adenosyl methonine synthase) was found up-regulated in floral flush, involved in the biosynthesis of polyamines has association with flowering, and stress responses. Furthermore, ARF (Auxin Response Factor), serine/threonine kinase gene members were also found to play critical role in determining floral development process in mango, consistent with results obtained through 2DE. Protein species that are putatively involved in phenylpropanoid pathway were also identified, showing the process of mango flowering from a new perspective beyond the conventional view. This flowering related proteomics study provides an overview of the biological pathways and regulatory mechanisms associated with flowering developmental physiology.
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