Papers by Ioanna Katsiadaki
Evolutionary Ecology Research, 2016
Background: The Arctic is subject to atmospheric deposition of persistent organic pollutants thro... more Background: The Arctic is subject to atmospheric deposition of persistent organic pollutants through the process of global distillation. It also contains thousands of sites with local sources of contamination, including military installations, mining operations, and petroleum extraction facilities. Pollutants accumulate in surface waters. Aim: To investigate the utility of ninespine stickleback (Pungitius pungitius) as a sentinel fish species to monitor the presence and biological effects of chemical contamination in the Arctic. Organism: Pungitius pungitius is a circumpolar species that occurs in freshwater, brackish water, and marine habitats. It is often the most abundant fish present. It appears to be relatively hardy with respect to potentially lethal effects of contaminants, which allows investigation of perturbed biological processes such as endocrine, gene expression, and developmental disruption. Biomarker development: We developed a homologous vitellogenin assay for the ni...
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Diseases of Aquatic Organisms
Enteric redmouth disease (ERM) caused by the enterobacterium Yersinia ruckeri poses a significant... more Enteric redmouth disease (ERM) caused by the enterobacterium Yersinia ruckeri poses a significant threat to salmonid aquaculture globally. Despite decades of experimental infection studies, key knowledge gaps remain regarding the onset of disease susceptibility and mechanisms of immunity during early developmental stages, undermining disease management efforts in all susceptible life-stages. In this study, a series of immersion challenges were conducted, challenging and re-challenging rainbow trout Oncorhynchus mykiss (Walbaum) at 7, 14 and 51 d post-hatch (dph; mean weights = 0.085, 0.1 and 2.0 g respectively) to high concentrations (1.72 × 107-1.1 × 108 CFU) of Y. ruckeri at 15°C. This study indicates the hitherto unknown initial point of susceptibility to infection as the time of first ingestion of exogenous food (14 dph), and shows that individuals surviving primary challenge at 14 dph are significantly more likely to survive re-challenge at 51 dph compared with naive individual...
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The potential of developing a biomarker for androgens in the three-spined stickleback (via the ki... more The potential of developing a biomarker for androgens in the three-spined stickleback (via the kidney glue protein that breeding males use to construct a nest) was recognised 10 years ago. As a consequence, under the UK EDMAR programme an ELISA for the only known so far androgen-regulated protein in fish, the stickleback glue spiggin, was developed and validated. More recently the stickleback assay was modified in two different ways to detect anti-androgens, a group of EDCs that poses an increasing risk to the aquatic environment as the in vitro data obtained from two nationwide surveys of final sewage effluents suggest. Importantly, the stickleback is present in Europe (and across all of the North hemisphere) and can bring together lab and field studies providing a sound basis for environmental risk assessment. To this end, the UK (Defra CN) has financed three research programmes that underpinned the background and validation data requirements that could lead to the inclusion of th...
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Scientific Reports, 2020
Model fish species such as sticklebacks and zebrafish are frequently used in studies that require... more Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and reliable to perform but that can harm fish. An alternative procedure to sample DNA involves swabbing the skin to collect mucus and epithelial cells. Although swabbing appears to be less invasive than fin clipping, it still requires fish to be netted, held in air and handled—procedures that can cause stress. In this study we combine behavioural and physiological analyses to investigate changes in gene expression, behaviour and welfare after fin clipping and swabbing. Swabbing led to a smaller change in cortisol release and behaviour on the first day of analysis compared to fin clipping. It also led to less variability in data suggesting that fewer animals need to be measured after using this technique. However, swabbing triggered some longer term changes in zebrafish behaviou...
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Evolutionary Ecology Research, 2016
Background: Female threespine sticklebacks, Gasterosteus aculeatus, are batch spawners. As in mos... more Background: Female threespine sticklebacks, Gasterosteus aculeatus, are batch spawners. As in most teleosts, the ovulated eggs are kept in the ovarian cavity until spawning. If spawning or spontane ...
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The 21-day androgenised female stickleback endocrine screening assay
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Scientific Reports, Jun 18, 2021
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Current Opinion in Environmental Science & Health, Oct 1, 2019
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Fish and Fisheries, 2016
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Marine biology, Dec 15, 2006
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Aquatic Toxicology, May 1, 2010
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The Journal of Steroid Biochemistry and Molecular Biology, 2017
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Scientific Reports, 2019
Development requires the implementation of a plethora of molecular mechanisms, involving a large ... more Development requires the implementation of a plethora of molecular mechanisms, involving a large set of genes to ensure proper cell differentiation, morphogenesis of tissues and organs as well as the growth of the organism. Genome duplication and resulting paralogs are considered to provide the raw genetic materials important for new adaptation opportunities and boosting evolutionary innovation. The present study investigated paralogous genes, involved in three-spined stickleback (Gasterosteus aculeatus) development. Therefore, the transcriptomes of five early stages comprising developmental leaps were explored. Obtained expression profiles reflected the embryo’s needs at different stages. Early stages, such as the morula stage comprised transcripts mainly involved in energy requirements while later stages were mostly associated with GO terms relevant to organ development and morphogenesis. The generated transcriptome profiles were further explored for differential expression of kno...
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Journal of fish biology, 2018
Wild fish populations are currently experiencing unprecedented pressures, which are projected to ... more Wild fish populations are currently experiencing unprecedented pressures, which are projected to intensify in the coming decades. Developing a thorough understanding of the influences of both biotic and abiotic factors on fish populations is a salient issue in contemporary fish conservation and management. During the 50th Anniversary Symposium of The Fisheries Society of the British Isles at the University of Exeter, UK, in July 2017, scientists from diverse research backgrounds gathered to discuss key topics under the broad umbrella of 'Understanding Fish Populations'. Below, the output of one such discussion group is detailed, focusing on tools used to investigate natural fish populations. Five main groups of approaches were identified: tagging and telemetry; molecular tools; survey tools; statistical and modelling tools; tissue analyses. The appraisal covered current challenges and potential solutions for each of these topics. In addition, three key themes were identified...
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Aquatic Toxicology, 2007
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Marine Biology, 2006
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Data in Brief, Dec 1, 2016
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Diseases of Aquatic Organisms, 2023
Enteric redmouth disease (ERM) caused by the enterobacterium Yersinia ruckeri poses a significant... more Enteric redmouth disease (ERM) caused by the enterobacterium Yersinia ruckeri poses a significant threat to salmonid aquaculture globally. Despite decades of experimental infection studies, key knowledge gaps remain regarding the onset of disease susceptibility and mechanisms of immunity during early developmental stages, undermining disease management efforts in all susceptible life-stages. In this study, a series of immersion challenges were conducted, challenging and re-challenging rainbow trout Oncorhynchus mykiss (Walbaum) at 7, 14 and 51 d post-hatch (dph; mean weights = 0.085, 0.1 and 2.0 g respectively) to high concentrations (1.72 × 107-1.1 × 108 CFU) of Y. ruckeri at 15°C. This study indicates the hitherto unknown initial point of susceptibility to infection as the time of first ingestion of exogenous food (14 dph), and shows that individuals surviving primary challenge at 14 dph are significantly more likely to survive re-challenge at 51 dph compared with naive individuals (hazard ratio = 1.446, p = 0.032). Other key findings include large variation in mortality between different development-stages, from 21.1% at 14 dph to 81.2% at 51 dph, and novel age-dependent symptoms not reported previously. Results from this study enhance our understanding of ERM in juvenile rainbow trout and inform the development of improved aquatic animal health management strategies, thereby contributing to the productivity and sustainability of salmonid aquaculture into the future.
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Papers by Ioanna Katsiadaki