Papers by Vasil G Georgiev
Springer eBooks, 2017
The biosynthesis of biologically active secondary metabolites in plant tissues is frequently rela... more The biosynthesis of biologically active secondary metabolites in plant tissues is frequently related to their differentiation. From this point of view, the hairy roots are a suitable plant in vitro system for producing bioactive substances due to their degree of differentiation and metabolic profiles identical to or similar to that of the root systems of the intact plants from which they are derived. In addition, they are grown in growth regulators-free nutrient media, which is essential for the subsequent application of their metabolites in variety of nutritional and pharmaceutical systems. They are characterized by high genetic and biochemical stability, making possible the scale-up of the cultivation processes in bioreactors. This provides complete control and management of the biosynthetic process and results in high yields of target metabolites for a short period of time, regardless of seasons. Salvia plant species are widely used in traditional medicine. The major biological activities they possessed are antioxidant, anti-inflammatory, anti-tumor activities and etc. These activities are consequence of biosynthesized biologically active substances: sterols, various polyphenol structures, di- and triterpenes, etc. Data about Salvia hairy roots in the scientific literature are limited. Nevertheless, it is clear that they biosynthesized mainly diterpenes and phenolic compounds. Current progress of investigation of Salvia hairy roots is limited to the phytochemical profiling and assessment of the influence of different independent variables (mainly elicitors) on the yields of the target bioactive substances and the development of commercial production process is not visible at the time. Therefore in the near future it is necessary an integrated approach for optimizing production processes to be developed. In our opinion, this is the basis for successful achievement of the commercially important yields of bioactive substances produced by hairy roots of Salvia species.
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The Journal of Microbiology, Biotechnology and Food Sciences, Jun 1, 2018
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Food Science and Applied Biotechnology, Mar 18, 2019
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Cytometry Part A, Oct 1, 2008
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Engineering in Life Sciences, Mar 1, 2021
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Springer eBooks, Jun 14, 2012
Since hairy root cultures of red beet were obtained at the end of the twentieth century, they hav... more Since hairy root cultures of red beet were obtained at the end of the twentieth century, they have been intensively investigated over the past decade as producers of biologically active compounds, including food colorants (betacyanin and betaxanthin pigments), phenolic compounds, enzymes and other proteins and for understanding basic cellular physiologies as well as for engineering their container designs, such as bioreactors. The analyses of available data concerning their growth potential, nutrient needs and secondary metabolite profiles outline these types of in vitro cultures as effective producers of biologically active colorants for the food and pharmaceutical industries, although commercial scales are yet to be realized. The main limitation for the development of such technologies is the cost-effectiveness of the process and the need for refinement of an appropriate bioreactor system suitable for further scale-up. This chapter summarizes the recent advances in bioreactor cultivation of beet hairy root cultures. The application of bioreactor systems with different designs, optimization of the cultivation conditions, monitoring and assessment of the cultivation process, methods for enhancement of pigment production and strategies for on-line product recovery are reviewed, compared and discussed with the purpose of assessing their effectiveness for the development of an effective industrial process. The advantages of biotechnologically produced red beet colorants, their possible applications and the law frame for their regulation are discussed as well.
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Proceedings of MOL2NET'22, Conference on Molecular, Biomedical & Computational Sciences and Engineering, 8th ed. - MOL2NET: FROM MOLECULES TO NETWORKS, Oct 21, 2022
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Planta Medica, Jul 1, 2008
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Journal of Bioscience and Bioengineering, Apr 1, 2009
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Acta Physiologiae Plantarum, Apr 15, 2008
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Engineering in Life Sciences, Sep 19, 2019
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Engineering in Life Sciences, Feb 22, 2010
The ploidy levels of the cells in different organs (leaves, petioles and roots) of red beet (Beta... more The ploidy levels of the cells in different organs (leaves, petioles and roots) of red beet (Beta vulgaris L.) plants of different ages, as well as of different in vitro systems (transformed hairy roots, calli derived from leaves and rhizogenic calli), were investigated using flow cytometry. Two callus lines with red and yellow phenotypes, derived by mechanical separation of the morphologically heterogeneous rhizogenic callus, were also examined. All investigated samples experienced several cycles of endoreduplication. The older organs exhibited higher levels of polysomaty than the young ones. The highest degree of endoreduplication was found in old petiole tissue and the lowest in the red callus line (cycle values of 1.81 and 0.55, respectively). Interestingly, the callus derived from leaves did not exhibit a 2Cx peak, but was tetraploid, probably due to genetic instability, which may have been caused by prolonged cultivation under in vitro conditions. Red and yellow calli showed significantly lower polysomaty (cycle values of 0.55 and 0.59, respectively) than the primary rhizogenic callus (cycle value of 1.09). The DNA profiles of the two phenotypes differed, possibly reflecting differences in their metabolism.
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Natural Product Communications, Mar 1, 2009
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Acta horticulturae, Apr 1, 2017
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Molecules, Dec 14, 2022
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Plants
Invasive species as sources of natural components are of increasing interest for scientific resea... more Invasive species as sources of natural components are of increasing interest for scientific research. This is the case of Ailanthus altissima, which belongs to the top 100 of the most dangerous invasive plant species in Europe, and which is the subject of the present study. The purpose of the research was to analyze the main phenolic compounds in the flowers, leaves, and stem bark of A. altissima and determine the DNA-protective and antioxidant potential of their ethanolic extracts. HPLC profiling revealed the presence of 6 flavonoids and 10 phenolic acids, of which 15 were found in flowers, 14 in leaves, and 11 in the stem bark. Rutin (5.68 mg/g dw in flowers), hesperidin (2.67 mg/g dw in leaves) and (+)-catechin (2.15 mg/g dw in stem bark) were the best-represented flavonoids. Rosmarinic (10.32 mg/g dw in leaves) and salicylic (6.19 mg/g dw in leaves) acids were predominant among phenolic acids. All plant extracts tested showed in vitro antioxidant activity (determined by DPPH, AB...
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Phytonutritional Improvement of Crops
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Papers by Vasil G Georgiev
Cellular agriculture is a multidisciplinary technology, exploring the potential of individual cells from plants and animals or microorganisms to produce agricultural products in controlled environment in bioreactors instead of relying on fields and farmed animals. Many novel foods and functional ingredients can be produced by large scale cultivation of cell cultures or precision fermentation without the need to use cultivated crops of farmed livestock. This technology is sustainable, environmentally friendly, requires significantly less energy, land and water use when compared to traditional agriculture practices.
This Article Collection aims to summarize the latest achievements in research and development of agricultural products, produced by cellular agriculture techniques. Possible topics include, but are not limited to:
• Mammalian cells for cellular agriculture
• Plant cells for cellular agriculture
• Microbial cells and algae for cellular agriculture
• Insect cells for cellular agriculture
• Precision fermentation
• Production and formulation of cellular and acellular products
• Production of foods by cellular agriculture
• Production of cosmetic ingredients by cellular agriculture
• Production of fibers, raw materials and functional ingredients by plant cells
• Tissue engineering, new matrix for cell encapsulation, 3D printing
• Process optimization and cost effectiveness
• Safety, legislation and public concerns
We welcome the submission of both original research and review papers.
Consumers' demand for high-quality and efficient cosmetics and personal care products continues to grow worldwide. In response, thousands of new products are launched on the market every year. To be viable, the new formulations have to comply with the highest quality standards, demonstrating proven activity, as well as international and national regulations. In addition, they have to be as natural as possible, being produced using sustainable and ecofriendly technology, having a low carbon footprint and recyclable packing, and being reasonably priced. Over the past decade, we have witnessed significant advances in the research into and development of cosmetics. Hundreds of new natural active ingredients originating from plants, microbes, algae, moss, snails, fish, and minerals have been developed and commercialized. With the advances in cellular agriculture, a lot of active substances, produced using plant cells and tissue culture technology, precision fermentation, and biotransformation have been introduced. New insights in natural deep eutectic solvent (NADES) research has allowed the application of highly efficient