Papers by Argelia Lorence
Plant Physiology and Biochemistry, May 1, 2013
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CRC Press eBooks, Jun 9, 2023
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Toxicon, Jun 29, 1998
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Phytochemistry, Oct 1, 2004
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In Vitro Cellular & Developmental Biology – Plant, Nov 21, 2008
... Khaleda L.; Al-Forkan M. Genotypic variability in callus induction and plant regeneration thr... more ... Khaleda L.; Al-Forkan M. Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deepwater rice ... doi:10.1007/s00299-004-0843-6. Naqvi SMS;Sultana T.; Yasmin T.; Mahmood T.; Akhtar MS Efficient embryogenic system from ...
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Plant Cell Reports, 2004
Camptothecin (CPT) is an anticancer and antiviral alkaloid produced by the Chinese tree Camptothe... more Camptothecin (CPT) is an anticancer and antiviral alkaloid produced by the Chinese tree Camptotheca acuminata (Nyssaceae) and some other species belonging to the families Apocynaceae, Olacaceae, and Rubiaceae. Bark and seeds are currently used as sources for the drug. Several attempts have been made to produce CPT from cell suspensions; however, the low yields obtained limit this approach. Cultures of differentiated cell types may be an alternative source of alkaloid production. Hairy root cultures of C. acuminata were established from tissue transformed with Agrobacterium rhizogenes strains ATCC 15834 and R-1000. Integration of the genes responsible for the hairy-root phenotype ( rol genes) into the plant genome was verified by DNA gel blot analysis. The hairy roots produce and secrete CPT as well as the more potent and less toxic natural derivative, 10-hydroxycamptothecin (HCPT), into the medium. Remarkably, the cultures were able to synthesize the alkaloids at levels equal to, and sometimes greater than, the roots in planta, i.e., 1.0 and 0.15 mg/g dry weight for CPT and the HCPT, respectively.
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In plants, l-ascorbic acid (AsA) is a functional enzyme cofactor, a major antioxidant, and a modu... more In plants, l-ascorbic acid (AsA) is a functional enzyme cofactor, a major antioxidant, and a modulator of several biological processes including photosynthesis, photo-protection, cell wall growth and expansion, tolerance to environmental stresses, and synthesis of other molecules. One of the major roles of AsA in plants is detoxifying reactive oxygen species (ROS) such as singlet oxygen or peroxide radicals. ROS are produced when plants undergo biotic or abiotic stresses and if accumulated in high concentrations, can cause damage to macromolecules such as nucleic acids, membrane lipids, and proteins. Until now, little study has been done on ascorbate metabolism in liverworts. Bryophytes (liverworts, hornworts, and mosses) comprise the earliest diverging land plant lineages that came about approximately 360–450 million years ago between the Ordovician and Devonian periods. The ancient liverwort Marchantia polymorpha is an emergent model system specifically suited to use in the study of the evolution of different biosynthetic pathways. In this chapter, basal levels of both reduced and oxidized AsA in M. polymorpha are reported. Comparative and functional genomics experiments in combination with precursor feeding experiment are also discussed in order to provide valuable insights on the evolution of the AsA biosynthetic pathways.
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Biotecnología, 1992
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Methods in molecular biology, 2022
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Ascorbic Acid in Plant Growth, Development and Stress Tolerance, 2017
l-Ascorbic acid (AsA, ascorbate or vitamin C) is the most abundant water-soluble antioxidant foun... more l-Ascorbic acid (AsA, ascorbate or vitamin C) is the most abundant water-soluble antioxidant found in plants. Ascorbate is synthesized via four pathways involving d-mannose/l-galactose, d-galacturonate, l-gulose, and myo-inositol as main precursors. In addition to protecting plant tissues from damage caused by reactive oxygen species produced through normal oxygenic metabolism or those generated from biotic and abiotic stresses, ascorbate is also an enzyme cofactor and a modulator of cell division, cell expansion, flowering time, and gene regulation. Plants that are deficient in ascorbate are affected in multiple ways including alterations in cell division, cell expansion, seed germination, growth, floral induction, and photosynthesis. Additionally, elevated ascorbate content in plants leads to an increase in their nutritive value, lengthening of their shelf life, enhancement on their growth rate and biomass accumulation, and to an increased tolerance to multiple abiotic stresses including salt, cold, heat, and water deficit. Increasing the productivity of crops is imperative to satisfy the growing demand for food, feed, and fuels in the world, and biotechnology can lead to the development of plants with higher yields capable of thriving under adverse conditions. To this end, phenotype screening and characterization of a large number of plants experimentally obtained is time consuming and requires a significant amount of resources, skills, and expertise. In this chapter, we will present an overview of how the use of high-throughput phenotyping or phenomics is revolutionizing the way plant phenotypes are characterized and will illustrate the power of digital phenotyping in the characterization of plants overexpressing enzymes in the inositol pathway to ascorbate.
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In plants, l-ascorbic acid (AsA) is a functional enzyme cofactor, a major antioxidant, and a modu... more In plants, l-ascorbic acid (AsA) is a functional enzyme cofactor, a major antioxidant, and a modulator of several biological processes including photosynthesis, photo-protection, cell wall growth and expansion, tolerance to environmental stresses, and synthesis of other molecules. One of the major roles of AsA in plants is detoxifying reactive oxygen species (ROS) such as singlet oxygen or peroxide radicals. ROS are produced when plants undergo biotic or abiotic stresses and if accumulated in high concentrations, can cause damage to macromolecules such as nucleic acids, membrane lipids, and proteins. Until now, little study has been done on ascorbate metabolism in liverworts. Bryophytes (liverworts, hornworts, and mosses) comprise the earliest diverging land plant lineages that came about approximately 360–450 million years ago between the Ordovician and Devonian periods. The ancient liverwort Marchantia polymorpha is an emergent model system specifically suited to use in the study ...
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PLOS ONE, 2019
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Plant Cell Reports, 2009
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Plant Physiology, 2019
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Methods in Molecular Biology
The advent of plant phenomics, coupled with the wealth of genotypic data generated by next-genera... more The advent of plant phenomics, coupled with the wealth of genotypic data generated by next-generation sequencing technologies, provides exciting new resources for investigations into and improvement of complex traits. However, these new technologies also bring new challenges in quantitative genetics, namely, a need for the development of robust frameworks that can accommodate these high-dimensional data. In this chapter, we describe methods for the statistical analysis of high-throughput phenotyping (HTP) data with the goal of enhancing the prediction accuracy of genomic selection (GS). Following the Introduction in Sec. 1, Sec. 2 discusses field-based HTP, including the use of unoccupied aerial vehicles and light detection and ranging, as well as how we can achieve increased genetic gain by utilizing image data derived from HTP. Section 3 considers extending commonly used GS models to integrate HTP data as covariates associated with the principal trait response, such as yield. Part...
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Biosynthesis of l-ascorbate (AsA) in plants is carried out by a complex metabolic network, which ... more Biosynthesis of l-ascorbate (AsA) in plants is carried out by a complex metabolic network, which involves d-mannose/l-galactose, d-galacturonate, l-gulose, and myo-inositol as main precursors. Arabidopsis lines over-expressing enzymes in the myo-inositol pathway have elevated AsA, accumulate more biomass of both aerial and root tissues, and are tolerant to abiotic stresses as shown by manual and digital phenotyping. We crossed myo-inositol oxygenase (MIOX4) over-expressers with two low-vitamin C mutants (vtc1-1 and vtc2-1) encoding enzymes involved in d-mannose/l-galactose route. The purpose of developing these crosses was to test MIOX4’s ability to restore the low AsA phenotype in mutants, and to assess the contribution of individual biosynthetic pathways to abiotic stress tolerance. We used a powerful high-throughput phenotyping platform for detailed phenotypic characterization of the Arabidopsis crosses with visible, fluorescence, near-infrared and infrared sensors. We combined d...
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New Phytologist
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Environmental pollution is a global concern that is threatening the well-being of all life forms ... more Environmental pollution is a global concern that is threatening the well-being of all life forms including humans. The cost of cleaning up contaminated sites is high and phytoremediation, the use of plants for removal of environmental pollutants, offers an attractive option due to its low cost and safety of implementation. The hairy roots technology has potential to become an excellent platform for studying numerous aspects encompassing phytoremediation. This is because hairy roots can be grown in large mass in culture media in a controlled environment and can therefore be subjected to various physiological assays. Also, these transformed roots are amenable to genetic manipulation and may facilitate the characterization of genes that influence the phytoremediation capacity of plants. This idea is well supported by the recent success in the development of transgenic plants for use in phytoremediation. Thus, hairy roots offer a good opportunity for the initial assessment of transgene ...
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Papers by Argelia Lorence