Cows become fail to conceive with various factors including management failures, nutritional stat... more Cows become fail to conceive with various factors including management failures, nutritional status, postpartum reproductive health, semen quality and other miscellaneous factor and hence reduce efficiency of AI service. Mainly heat detection skill by farmers and timing of insemination are the major factors that determine the success and failure of AI programme. Am/Pm rule is the way which helps to determine relative insemination times achieved in practice, since maximum fertility to artificial insemination occurs when cows are bred near the end of "standing heat". Ovulation occurs about 12 hours after the end of standing heat. Management limitations also synergize other factors like delivery problems which prone the AI service to have inefficient and poor result. Conservative stocking rate, a sensible year round feeding and herd health plan and adequate AI service are important to improve reproductive efficiency, and hence, economically benefit from the crossbreeding acti...
Response to the superovulatory hormone treatments varied for the type of breed and doses of super... more Response to the superovulatory hormone treatments varied for the type of breed and doses of superovulatory hormone used. Ovarian response of 42 cross bred HF (50%, 75%) and pure dairy cows were evaluated based on ultrasonographic count of CL and embryo recovery for quality assessment. Ovarian response rate was significantly higher (P<0.05) in the crossbreds than in the pure bred cows. The maximum response achieved (CL count, mean ± SE) was 14.6±1.1 and the least with 11.2±1.1, 75% HF at 650IU and pure HF at 800IU, respectively. Significantly higher (p<0.05) number of UFO were collected at a dose of 500 IU while transferable embryos were higher at 650IU in both the 50% HF and 75% HF cows. Pure bred cows responded fairly with more CL and produced higher number of transferable embryos at a dose of 800 IU FSH. In conclusion, the optimal dose for maximum response (CL count) exhibited in cross breed HF cows is 650 IU.
The variation of the dairy breed can determine the success of bovine embryo transfer by influenci... more The variation of the dairy breed can determine the success of bovine embryo transfer by influencing the quantity and quality of in vivo embryo production. In this experiment, output and quality of in vivo produced embryos using semen of progeny tested Holstein Friesian (HF) sire in Boran and HF*Boran F1 cross cows, and semen from purebred Boran sire in HF*Boran F1 cross and Boran cows were evaluated. Boran (n=18) and HF*Boran cross (n=18) breed donor dams were superovulated using a previously optimized follicular stimulating hormone (FSH) (Pluset®) dose regimen: 650 IU for HF*Boran cross and 250 IU for Boran breeds. Each cow was flushed on Day-7 post insemination and embryos were evaluated for their developmental stages and quality. Superovulatory response rates were 88.9% and 83.3%, respectively, for Boran and HF*Boran with no significant (P>0.05) breed differences. Total recovery rates were relatively lower (56.5%) in Boran compared to in HF*Boran (67.4%). The mean (±SE) embr...
Cows become fail to conceive with various factors including management failures, nutritional stat... more Cows become fail to conceive with various factors including management failures, nutritional status, postpartum reproductive health, semen quality and other miscellaneous factor and hence reduce efficiency of AI service. Mainly heat detection skill by farmers and timing of insemination are the major factors that determine the success and failure of AI programme. Am/Pm rule is the way which helps to determine relative insemination times achieved in practice, since maximum fertility to artificial insemination occurs when cows are bred near the end of "standing heat". Ovulation occurs about 12 hours after the end of standing heat. Management limitations also synergize other factors like delivery problems which prone the AI service to have inefficient and poor result. Conservative stocking rate, a sensible year round feeding and herd health plan and adequate AI service are important to improve reproductive efficiency, and hence, economically benefit from the crossbreeding acti...
Response to the superovulatory hormone treatments varied for the type of breed and doses of super... more Response to the superovulatory hormone treatments varied for the type of breed and doses of superovulatory hormone used. Ovarian response of 42 cross bred HF (50%, 75%) and pure dairy cows were evaluated based on ultrasonographic count of CL and embryo recovery for quality assessment. Ovarian response rate was significantly higher (P<0.05) in the crossbreds than in the pure bred cows. The maximum response achieved (CL count, mean ± SE) was 14.6±1.1 and the least with 11.2±1.1, 75% HF at 650IU and pure HF at 800IU, respectively. Significantly higher (p<0.05) number of UFO were collected at a dose of 500 IU while transferable embryos were higher at 650IU in both the 50% HF and 75% HF cows. Pure bred cows responded fairly with more CL and produced higher number of transferable embryos at a dose of 800 IU FSH. In conclusion, the optimal dose for maximum response (CL count) exhibited in cross breed HF cows is 650 IU.
The variation of the dairy breed can determine the success of bovine embryo transfer by influenci... more The variation of the dairy breed can determine the success of bovine embryo transfer by influencing the quantity and quality of in vivo embryo production. In this experiment, output and quality of in vivo produced embryos using semen of progeny tested Holstein Friesian (HF) sire in Boran and HF*Boran F1 cross cows, and semen from purebred Boran sire in HF*Boran F1 cross and Boran cows were evaluated. Boran (n=18) and HF*Boran cross (n=18) breed donor dams were superovulated using a previously optimized follicular stimulating hormone (FSH) (Pluset®) dose regimen: 650 IU for HF*Boran cross and 250 IU for Boran breeds. Each cow was flushed on Day-7 post insemination and embryos were evaluated for their developmental stages and quality. Superovulatory response rates were 88.9% and 83.3%, respectively, for Boran and HF*Boran with no significant (P>0.05) breed differences. Total recovery rates were relatively lower (56.5%) in Boran compared to in HF*Boran (67.4%). The mean (±SE) embr...
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