Tilt pair validation server
Welcome to the PDBe tilt pair validation server!
Tilt-pair validation analysis (Rosenthal
and Henderson, 2003) can be used to assess the accuracy of initial angle assignment in
single-particle processing. To perform this analysis
you need to collect two corresponding sets of particle images - one untilted and the other tilted, then
upload the stacks of images along with a 3D reconstruction
based on the untilted images. This server is based on the Tilt-pair
server developed at MRC National Institute for Medical Research (Wasilewski and Rosenthal, 2014),
and we thank Sebastian Wasilewski and Peter Rosenthal for their help in developing and testing the
current server.
You may upload map files in MRC or CCP4 format, and parameter files (containing Euler angles for
individual particles) in Spider or Frealign format.
We have some test data sets that you can use to try out the service
here. We are still developing the
server and appreciate
your
feedback!
Quick links
Recent Entries
(Show all)HIV-1 BG505 Env SOSIP in complex with bovine Fab Bess4 and non-human primate Fab RM20A3
Toxoplasma gondii apical end with ICMAP3i conditionally knocked down
Toxoplasma gondii apical end with ICMAP1 conditionally knocked down
Toxoplasma gondii apical end with ICMAP2 conditionally knocked down
Cryo-EM structure of TRNM-f*01 Fab in complex with HIV-1 Env trimer ConC SOSIP
Cryo-EM structure of human claudin-4 complex with Clostridium perfringens enterotoxin, sFab COP-1, and Nanobody
Structure of biofilm-forming functional amyloid PSMa1 from Staphylococcus aureus
Cryo-EM structure of FoxA1 in complex with ALBN1 nucleosome (class 2)
Cryo-EM structure of TRNM-b*01 Fab in complex with HIV-1 Env trimer BG505.DS SOSIP
Cryo-EM structure of FoxA1 and GATA4 in complex with H14 chromatosome
Cryo-EM structure of FoxA1 in complex with ALBN1 nucleosome (class 1)
Crosslinked 6-deoxyerythronolide B synthase (DEBS) Module 3 in complex with antibody fragment 1B2: cis-oriented 1B2 and ACP
Cryo-EM structure of FoxA1 and GATA4 in complex with ALBN1 nucleosome
Cryo-EM structure of HERH-b*01 Fab in complex with HIV-1 Env trimer BG505.DS SOSIP
Cryo-EM structure of HIV-1 Env BG505 DS-SOSIP in complex with antibody GPZ6-b.01 targeting the fusion peptide
Crosslinked 6-deoxyerythronolide B synthase (DEBS) Module 3 in complex with antibody fragment 1B2: trans-oriented 1B2 and ACP
The conventional reconstruction of the MC-40 de novo processed HA-trimer
Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by activity-based chemical trapping strategy (adjacent H2AK13/15 dual-monoubiquitination)
The conventional reconstruction of the MC-45 de novo processed PS1
Cryo-EM structure of noradrenaline transporter in complex with bupropion
Salmonella enterica serovar Typhimurium FliC(G426A)delta(204-292) forming the L-type straight filament
Ebola virus glycoprotein in complex with a broadly neutralizing antibody 2G1
Cryo-EM structures of RNF168/UbcH5c-Ub/nucleosomes complex determined by activity-based chemical trapping strategy
Cryo-EM structure of noradrenaline transporter in complex with a x-MrlA analogue
Conventional Reconstruction of the MC-45 de novo processed ribosome 50S
Cryo-EM structure of human CCR8 in the inactive form at 3.15 angstrom resolution from Biortus
CryoEM structure of LonC protease S582A open pentamer with lysozyme
Cryo-EM structure of noradrenaline transporter in complex with ziprasidone
CryoEM structure of LonC protease S582A open hexamer with lysozyme
Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by intein-based E2-Ub-NCP conjugation strategy
Partially closed Falcilysin bound to MK-4815, from MK-4815-treated dataset
Cryo-EM map of RNF168/UbcH5c-Ub/nucleosome determined by E2-Ub-NCP conjugation strategy
Cryo-EM structure of noradrenaline transporter in complex with noradrenaline
Cryo-EM structure of human ASCT2 (SLC1A5) at 2.53 angstrom resolution from Biortus
CryoEM structure of LonC protease open pentamer in presence of AGS
cryo-EM map of RNF168(1-193) in complex with Ubc5c-Ub conjugated nucleosome at a resolution of 3.23 angstrom
Cryo-EM structure of human C-terminally bound ATG9A-ATG2A-WIPI4 complex
Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes (two Ub conformation)
Human Tip60 complex locally refined on ACTIN and the ATPase domain of EP400
Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
CryoEM Structure of Phenylalanine Ammonia Lyase from Planctomyces brasiliencis
Human DNA polymerase epsilon bound to DNA and PCNA (ajar conformation)
Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Human DNA Polymerase epsilon bound to T-C mismatched DNA (Post-Insertion state)
Human DNA polymerase epsilon bound to DNA and PCNA (closed conformation)
Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Human DNA Polymerase epsilon bound to T-C mismatched DNA (Polymerase Arrest state)
Human DNA Polymerase epsilon bound to T-C mismatched DNA (Mismatch Excision state)
Structure of the C. elegans Intron Lariat Spliceosome double-primed for disassembly (ILS'')
Human DNA Polymerase epsilon bound to T-C mismatched DNA (Frayed Substrate state)
Chlorella sorokiniana Rubisco with CsLinker (alpha3-alpha4) bound: D4 symmetry expanded
Composite map of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')
Human DNA polymerase epsilon bound to DNA and PCNA (open conformation)
Toxoplasma gondii apical end with ICMAP3ii conditionally knocked down