Abstract
The 21-base pair repeat elements of the SV40 promoter contain six tandem copies of the GGGCGG hexanucleotide (GC-box), each of which can bind, with varying affinity, to the cellular transcription factor, Sp1. In vitro SV40 early RNA synthesis is mediated by interaction of Sp1 with GC-boxes I, II, and III, whereas transcription in the late direction is mediated by binding to GC-boxes III, V, and VI.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Autoradiography
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Base Sequence
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Binding Sites
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DNA-Binding Proteins / metabolism*
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Deoxyribonuclease I / metabolism
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation
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Mutation
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Pregnancy Proteins / metabolism*
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RNA, Messenger / analysis
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RNA, Viral / biosynthesis
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Simian virus 40 / genetics*
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Sp1 Transcription Factor
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Templates, Genetic
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Pregnancy Proteins
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RNA, Messenger
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RNA, Viral
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Sp1 Transcription Factor
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Transcription Factors
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Deoxyribonuclease I