Abstract
Polyphenols are natural compounds of high structural diversity which translates into a very wide spectrum of biological activities, including chemoprevention. Here we report that a Japanese quince fruit flavanol preparation (JQFFP) caused favorable changes in Bax/Bcl-2 mRNA ratio, which rendered normal and cancer cells more resistant and more sensitive, respectively, to apoptosis. DU145 human prostate cancer cells were characterized by the most advantageous Bax/Bcl-2 ratio. The growth and invasiveness of MDA-MB-231 human breast cancer cells were strongly suppressed by JQFFP, which was accompanied with a decrease in MMP-9 activity and stimulation of TIMP-1 expression. Importantly, JQFFP did not decrease normal human prostate PNT1A cell number, whereas Bax/Bcl-2 ratio decreased which implies increased resistance to apoptosis. In conclusion, JQFFP exhibited a potent antiproliferative effect against cancer cells, inhibited their invasiveness, and decreased expression level of several genes involved in apoptosis, angiogenesis, and metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / analysis
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Antineoplastic Agents, Phytogenic / chemistry*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Apoptosis / drug effects
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Breast Neoplasms / drug therapy*
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Breast Neoplasms / genetics
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Breast Neoplasms / pathology
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Cell Line, Tumor
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Female
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Flavonoids / chemistry
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Flavonoids / pharmacology*
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Fruit / chemistry
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Male
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Matrix Metalloproteinase 9 / metabolism
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Neovascularization, Pathologic / genetics
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Phenols / analysis
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Phenols / chemistry
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Prostatic Neoplasms / drug therapy*
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / pathology
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Proto-Oncogene Proteins c-bcl-2 / genetics*
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Rosaceae / chemistry*
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Tissue Inhibitor of Metalloproteinase-1 / metabolism
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bcl-2-Associated X Protein / genetics*
Substances
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Antineoplastic Agents, Phytogenic
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BAX protein, human
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Flavonoids
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Phenols
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Proto-Oncogene Proteins c-bcl-2
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TIMP1 protein, human
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Tissue Inhibitor of Metalloproteinase-1
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bcl-2-Associated X Protein
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Matrix Metalloproteinase 9