Abstract
Here we firstly investigated the role of miR-138 in multidrug resistance of leukemia cells. miR-138 was found up-regulated in the vincristine-induced multidrug resistance (MDR) leukemia cell line HL-60/VCR as compared with HL-60 cells. Up-regulation of miR-138 could reverse resistance of both P-glycoprotein-related and P-glycoprotein-non-related drugs on HL-60/VCR cells, and promote adriamycin-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of adriamycin. miR-138 could significantly down-regulate the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1. Further study of the biological functions of miR-138 might be helpful for developing possible strategies to treat leukemia.
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
-
Antibiotics, Antineoplastic / pharmacology
-
Antineoplastic Agents, Phytogenic / pharmacology
-
Apoptosis / drug effects*
-
Blotting, Northern
-
Blotting, Western
-
Cell Line, Tumor
-
Doxorubicin / pharmacology
-
Drug Resistance, Multiple / genetics*
-
Drug Resistance, Neoplasm / genetics
-
Gene Expression Regulation, Leukemic / genetics*
-
Humans
-
Leukemia / drug therapy*
-
Leukemia / genetics*
-
Leukemia / pathology
-
Luciferases / metabolism
-
MicroRNAs / physiology*
-
Multidrug Resistance-Associated Proteins / genetics
-
Multidrug Resistance-Associated Proteins / metabolism
-
Vincristine / pharmacology
Substances
-
ATP Binding Cassette Transporter, Subfamily B, Member 1
-
Antibiotics, Antineoplastic
-
Antineoplastic Agents, Phytogenic
-
MIRN138 microRNA, human
-
MicroRNAs
-
Multidrug Resistance-Associated Proteins
-
Vincristine
-
Doxorubicin
-
Luciferases