Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis

Appl Environ Microbiol. 1992 Feb;58(2):709-12. doi: 10.1128/aem.58.2.709-712.1992.

Authors

Affiliation

¹ Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706-1187.

Abstract

Clamped homogeneous electric field analysis of Listeria DNA with ApaI, AscI, SmaI, or NotI revealed species- and serotype-specific differences in genomic fingerprints. Clamped homogeneous electric field analysis may prove useful, therefore, in epidemiologic studies. Also, the summation of individually sized AscI fragments of genomic DNA from L. monocytogenes serotype 4b 101M and Scott A indicated genome lengths of 2,925 and 3,046 kb, respectively.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA Fingerprinting
Electrophoresis, Gel, Pulsed-Field
Genome, Bacterial
Listeria / classification
Listeria / genetics
Listeria / isolation & purification*
Listeria monocytogenes / classification
Listeria monocytogenes / genetics
Listeria monocytogenes / isolation & purification*
Serotyping