Abstract
Z39Ig, a recently identified immunoglobulin (Ig) superfamily member, is localized in the pericentromeric region of human chromosome X and detectable in all human tissue, but it is predominantly expressed in fetal human tissues as well as in adult lungs and placenta. In the present study, we generated a monoclonal antibody against Z39Ig protein to investigate the immunological role of Z39Ig protein on various immune cells. The anti-Z39Ig mAb that we generated specifically bound to Z39Ig protein on human promonocytic THP-1 cells, monocytes isolated from human peripheral blood mononuclear cells (PBMC) and mature CD14(+) dendritic cells (DC) differentiated from umbilical-cord blood CD34(+) hematopoietic progenitor cells. In addition, a signal through the Z39Ig protein induced an obvious cell surface expression of HLA-DR on THP-1 cells mediated by MHC class II transactivator (CIITA). These data suggest that the Z39Ig protein might be a critical molecule to regulate an immune response mediated by phagocytosis and/or antigen presentation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / pharmacology*
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Antigens, CD34 / immunology
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DNA, Complementary / biosynthesis
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Female
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HLA-DR Antigens / biosynthesis
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HLA-DR Antigens / immunology
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HeLa Cells
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Humans
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Immunoglobulins / genetics
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Immunoglobulins / immunology
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Immunoglobulins / metabolism*
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Interferon-gamma / biosynthesis
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Jurkat Cells
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Lipopolysaccharide Receptors / immunology
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Lipopolysaccharides / pharmacology
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Lymphocytes / drug effects
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Lymphocytes / immunology
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Mice
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Mice, Inbred BALB C
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Monocytes / drug effects
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Monocytes / immunology
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Nuclear Proteins / biosynthesis
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Receptors, Complement
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Trans-Activators / biosynthesis
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Transfection
Substances
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Antibodies, Monoclonal
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Antigens, CD34
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DNA, Complementary
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HLA-DR Antigens
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Immunoglobulins
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Lipopolysaccharide Receptors
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Lipopolysaccharides
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MHC class II transactivator protein
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Nuclear Proteins
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Receptors, Complement
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Trans-Activators
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VSIG4 protein, human
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Interferon-gamma