|
| Status |
Public on Jan 27, 2009 |
| Title |
miR-124 transfection lysate RNA, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
miR-124 transfection 1, total RNA
|
| Organism |
Homo sapiens |
| Characteristics |
FLPin HEK 293 cells stably transfected with FLAG/HA-AGO2
|
| Treatment protocol |
FLAG/HA-AGO2 was immunoprecipitated with anti-FLAG M2 agarose
|
| Growth protocol |
HEK293 cells were grown in DMEM suppl. with FBS and 2mM Glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After proteinase K treatment extraction of total RNA was performed using Trizol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix One-Cycle Eukaryotic Target Labeling Assay protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from total RNA of mock-transfected cells
|
| Data processing |
CEL files were imported into R / BioConductor using the affy package. The raw intensities were corrected using gcRMA. The 18 arrays were finally quantile-normalized.
|
| |
|
| Submission date |
Jan 23, 2009 |
| Last update date |
Jan 26, 2009 |
| Contact name |
Markus Landthaler |
| E-mail(s) |
landthm@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14537 |
Contribution of sequence and structure to mRNA-binding of Argonaute/miRNA complexes and degradation of miRNA targets |
|
